Figure 7

Cell death occurs in small, focal areas after blast wave exposure. (A) A schematic representing an enface view of the retina showing the average number and distribution of affected (TUNEL-positive) areas (red bars) detected in retinal cross-sections collected in serial through the eye three days post-blast wave exposure. (B) Montage of low magnification epifluorescence micrographs of a retina three days post-blast wave exposure. White boxes indicate affected areas (scale bar is 250 μm). (C-E) Higher magnification epifluorescence micrographs of TUNEL (red) and DAPI (blue) in a control retina (C), and affected (D) and unaffected (E) regions of the retina shown in (B). The scale bar in (C) represents 50 μm and also applies to (D) and (E). ON = optic nerve head; GCL = ganglion cell layer; INL = inner nuclear layer; ONL = outer nuclear layer.