Figure 3

F-actin structure in rat astrocytes (confluent culture) treated with S1P, recombinant human plasma gelsolin and FTY720P, or their combination. At 8 hours incubation the cells were fixed with 4% paraformaldehyde, permeabilized with Triton X-100 and F-actin was stained with Phalloidin-FITC. Arrows indicate gaps in astrocyte monolayers. Data from one representative experiment are shown (A). B shows quantitative analysis of F-actin fluorescence in the astrocyte monolayer under treatment, indicated by numbers 1 to 10 in the left lower corner of each picture. *Significantly different. Error bars represent standard error of the mean. S1P - sphingosine – 1- phosphate, GSN - recombinant human plasma gelsolin.