Figure 7

An intraocular injection of TNFΑ causes nuclear accumulation of JUN in Müller cells. (A-C) PBS injections revealed a baseline accumulation of JUN in the ganglion cell layer (GCL) with minimal activation in the inner nuclear layer (INL). (D-F) A single injection of TNFΑ resulted in widespread accumulation of JUN (red) in SOX2-positive Müller cells (green) as early as 7 hours after treatment. A subset of SOX2-positive cells were negative for JUN and exhibited rounded nuclei and no processes that may be amacrine cells (F, arrows). Additionally, in the GCL a subset of SOX2+ cells also exhibited JUN nuclear accumulation and may be astrocytes. (G-I) Most of the Müller cells responded to TNFΑ with an increase in labeling for JUN accumulation, but only a subset of these also upregulated NFΚB (arrow). Cells were counterstained with 4’,6-diamidino-2-phenylindole (blue, I). After injection, sections were analyzed at 7 hours (n =3; A-F), and 24 hours (n =3; G-I). Scale bars (F) =50 μm and (I) =10 μm.