The effect of anti- TNF therapy on microvesicle counts and size. (A) Estimations of the total numbers of microvesicles after focal cerebral ischemia showing altered counts with anti-TNF therapy. (B) Estimation of the mean diameter of microvesicles after focal cerebral ischemia. (C) Correlation analysis of the total count of microvesicles and the infarct size at six hours (top), 24 hours (middle) and five days (bottom) after focal cerebral ischemia showing a significant correlation in saline-treated mice at 24 hours. Area fill for 24 hours is shown in grey (*P <0.05; **P <0.01; ***P <0.001, ****P <0.0001, Pearson r correlation analysis and one-way ANOVA, followed by Bonferroni post-hoc test; three to 24 per group). mm, millimeter; nm, nanometer; TNF, tumor necrosis factor.