Depletion of retinal dendritic cell (DC) eliminates the local immune response. (A) Schematic showing timing and location of diphtheria toxin (DTx) (25 ng), beta-galactosidase (βgal) (10 μg), and saline injections. (B) Fluorescence-activated cell sorting (FACS) analysis showing reduction in T cell numbers in retinas depleted of DC. T cells were analyzed as being effector (GFP−) or regulatory (GFP+) and βgal-specific (Vα11+) or nonspecific (Vα11−). (C) FACS analysis of retinal mononuclear cells showing that DC but not microglia (MG) were reduced in mice treated with DTx. Results are given as mean ± SD with P values determined by t test.