Figure 5From: Retinal antigen-specific regulatory T cells protect against spontaneous and induced autoimmunity and require local dendritic cells Depletion of retinal dendritic cell (DC) eliminates the local immune response. (A) Schematic showing timing and location of diphtheria toxin (DTx) (25 ng), beta-galactosidase (βgal) (10 μg), and saline injections. (B) Fluorescence-activated cell sorting (FACS) analysis showing reduction in T cell numbers in retinas depleted of DC. T cells were analyzed as being effector (GFP−) or regulatory (GFP+) and βgal-specific (Vα11+) or nonspecific (Vα11−). (C) FACS analysis of retinal mononuclear cells showing that DC but not microglia (MG) were reduced in mice treated with DTx. Results are given as mean ± SD with P values determined by t test.Back to article page