Figure 4

Apparent conversion of NG2 cell into an astrocyte cell in the substantia nigra pars reticulata (SNr) at day 30 after 6-hydroxydopamine (6-ODHA) lesion. Representative confocal micrographs showing nuclei stained with Hoechst (blue) and immunostaining against NG2 (green), and GFAP (red). Individual micrographs of NG2 and GFAP (top row), merged images of Hoechst/NG2/GFAP and NG2/GFAP (middle row) and vertical 1-μm optical slices for the cell indicated by the asterisk (bottom row) are shown. The scale bar = 20 μm is common for all the micrographs. GFAP, glial fibrillary acidic protein; NG2, Neuron/Glial type 2.