Figure 4
Helminth soluble factors inhibit agonist-induced TRPC1/ORAI1 store-operated Ca 2+ entry. Microglial cells were used for electrophysiological recordings, and both lipopolysaccharide (LPS)- and thapsigargin (Tg)-induced currents were evaluated in control cells and cells treated with helminth soluble factor (HSF) (25 μg/ml for 20 minutes). (A) Inward currents were induced upon addition of LPS (10 ng/ml). The holding potential for current recordings was −80 mV in both control and HSF-treated cells. (B) Respective current-voltage (I-V) curves using the ramp protocol in control and HSF-treated cells stimulated with LPS are shown. (C) Average of eight to ten recordings with current intensity at −80 mV are shown. (D) Inward currents induced by the addition of Tg (1 μM) in control cells and cells pretreated with HSF (25 μg/ml for 20 minutes). (E) The respective I-V curves under these conditions are shown. (F) Average of eight to ten recordings of current intensity at −80 mV are shown. *P ≤ 0.05.