Figure 3

Effects of pretreatment of ouabain (OUA) (1.8 μg/ kg) on lipopolysaccharide (LPS)-induced (200 μg/kg ) p65 (RELA) subunit NF- κB nuclear translocation and IκB (IKB) degradation. Pretreatment of OUA blocks the LPS-induced NF-κB activation. (A) Representative Western blotting autoradiographs and densitometric analysis (arbitrary units, AU) of p65 nuclear/β-ACTIN ratio, *P < 0.05 versus control and OUA + LPS. (B) Representative Western blotting autoradiographs and densitometric analysis (arbitrary units, AU) of IKB cytosolic/β-ACTIN ratio, *P < 0.05 versus control, OUA, OUA + LPS. Data are presented as mean ± SEM from five individual experiments. One-way ANOVA followed by Newman-Keuls post-test.