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Figure 6 | Journal of Neuroinflammation

Figure 6

From: Activation of toll like receptor 4 attenuates GABA synthesis and postsynaptic GABA receptor activities in the spinal dorsal horn via releasing interleukin-1 beta

Figure 6

Lipopolysaccharide (LPS) suppresses glial glutamate transporter activities through releasing IL-1β and activating protein kinase C (PKC). Glutamate transporter currents (GTCs) were recorded from glial fibrillary acidic protein-GFP (GFAP-GFP) mice. Samples of GTCs recorded before (baseline), during and after washout of LPS (1 μg/ml) are shown in (A). Samples of GTCs recorded before (baseline), during perfusion of LPS (1 mg/ml) in the presence of IL-1β receptor blocker (IL-1ra, 100 ng/ml) (B) or in the presence of the PKC inhbitor, GF109203X (GF, 4 μM) (C) are shown. Samples of GTCs recorded before (baseline), during perfusion of LPS (1 mg/ml) with a recording pipette filled with the intracellular solution containing the PKC inhibitor (PKCI 19–30, 5 μM) are shown in (D). Bar graphs show the mean (+SE) amplitude and charge transfer of GTCs before (baseline), during and after washout of tested agents. ***P < 0.001; NS: no statistical significance.

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