Figure 5From: Chemokine-mediated inflammation in the degenerating retina is coordinated by Müller cells, activated microglia, and retinal pigment epithelium In situ hybridization for Cxcl1 mRNA in the retina following exposure to 24 hours of light damage. A: In sections from dim-reared animals, staining for Cxcl1 was absent. B-D: Retinas exposed to 24 hrs of LD showed staining for Cxcl1 in putative retinal pigment epithelium (RPE) cells (arrowheads) in the superior retina (B-C), and few-to-none in the inferior (D). E-F: Staining for Cxcl1 mRNA was also present in the inner nuclear layer (INL) of superior retina following 24 hrs of LD (E; arrowheads), and mostly absent in inferior retina (F). G-H: Retinas with stain for Cxcl1 mRNA in the INL (red; arrowhead), which was found to co-localize with vimentin-immunolabeled Müller cell processes (green; arrowheads). I-J: Localized Cxcl1 stain in putative RPE cells (red; arrowheads) correlated strongly with immunolabeling for RPE65 (green; arrowheads); Cxcl1 stain appeared to be localized in the nucleus, rather than cytoplasm. C, choroid; INL, inner nuclear layer; IHC, immunohistochemistry; ISH, in situ hybridization ONL, outer nuclear layer; OS, outer segments.Back to article page