Table 1 Primers for RT-PCR
From: Neuromelanin activates proinflammatory microglia through a caspase-8-dependent mechanism
Target mRNA | Forward (F) and reverse (R) primers |
|---|---|
TLR4 | F: 5-GGA CTC TGA TCA TGG CAC TG-3 |
R: 5-CTG ATC CAT GCA TTG GTA GGT-3 | |
TLR2 | F: 5-GGG GCT TCA CTT CTC TGC TT-3 |
R: 5-AGC ATC CTC TGA GAT TTG ACG-3 | |
CD14 | F: 5-AAA GAA ACT GAA GCC TTT CTC G-3 |
NOD1 | R: 5-AGC ACC AAG CCA AGC ACA C-3 |
NOD2 | F: 5-AGT GAG GGC GGG AAG TGT-3 |
R: 5-CCA ATC TGA TTA CCC CAC ATC-3 | |
F: 5-CCC TAG CAC TGA TGC TGG A-3 | |
R: 5-CCC CTT CGT CAC AGA TAT GG-3 | |
RAGE | F: 5-GTG TCG GGC AAC TAA CAG G-3 |
R: 5-CTG GCT TCC CAG GAA TCT G-3 | |
IL-6 | F: 5-TTC TTG GGA CTG ATG CTG-3 |
R: 5-CTG GCT TTG TCT TTC TTG TT-3 | |
IL-1β | F: 5-TTG ACG GAC CCC AAA AGA TG-3 |
R: 5-AGA AGG TGC TCA TGT CCT CA-3 | |
iNOS | F: 5-CTT TGC CAC GGA CGA GAC-3 |
R: 5-TCA TTG TAC TCT GAG GGC TGA C-3 | |
TNF-α | F: 5-AGC CCA CGT AGC AAA CCA CC-3 |
R: 5-AAC ACC CAT TCC CTT CAC AGA GC-3 | |
GPDH | F: 5-AAC GAC CCC TTC ATT GAC C-3 |
R: 5-TCA GAT GCC TGC TTC ACC-3 |