The expression levels of C1qa and C3 were reduced in GW0742-treated mouse brain as analyzed by immunohistochemistry. Immunohistochemistry against C1qa and C3 revealed a staining pattern associated predominantly with glial fibrillary acidic protein (GFAP) immunoreactivity. Figures (A) and (B) show typical example images of C1qa and GFAP immunoreactivities in vehicle-treated TG mice, respectively and figure (C) is the overlay of C1qa and GFAP. Figures (D-F) show example images of corresponding immunoreactivities in GW0742-treated TG mice. Figures (G-H) show C3 and GFAP immunoreactivities in vehicle-treated TG mice and figure (I) the overlay of C3 and GFAP. Figure (J-L) depict example images of corresponding immunoreactivities in GW0742-treated TG mice. The high magnification insets show typical C1qa and C3 staining pattern resembling astrocytes. Scale bar = 200 μm.