Figure 9

GW0742 prevented the inflammation-induced neuron death in vitro . Neurons were cultivated in the presence of microglia and pre-exposed to vehicle or GW0742. Thereafter, the cells were exposed 100 ng/ml LPS/30 ng/ml of IFN-γ for 48 hours in the presence of GW0742 or vehicle and the neuron loss analyzed by quantifying the extent of MAP-2 staining. GW0742 prevented inflammation-induced loss of MAP-2 immunoreactivity (A). Panels (B-D) show typical examples of vehicle (B), vehicle + LPS/IFN-γ (C) and LPS/IFN-γ + GW0742- (D) treated cells. Results are presented as mean ± SEM. VEH = vehicle- and GW0742 = GW0742-treated cells. **P < 0.01 ***P < 0.001 as analyzed by 1-way ANOVA followed by Tukey’s post hoc test. n = 3 to 4 (individual experiments) per group. Scale bar = 200 μm.