Figure 2
Comparison of experimental autoimmune uveoretinitis clinical and histopathological score in C57Bl/6 J Cx 3 cr1 GFP/+ mice. No adjuvant controls developed experimental autoimmune uveoretinitis (EAU). EAU clinical disease score (A) in C57Bl/6 J Cx 3 cr1 GFP/+ mice revealed a grade 2.8 disease by day 35 (d35) post-immunization (p.i.) (adjuvant controls: d0, d14, and d28: n = 14, d21 and 35: n = 24; IRBP1–20: d0: n = 33, d14: n = 24, d21: n = 32, d28: n = 20, d35: n = 28). (B) Correlation between total histopathological disease score in C57Bl/6 J Cx 3 cr1 GFP/+ mice on d35 p.i. and the clinical grade in the same mice (IRBP1–20: n = 8). Pearson’s correlation was applied to analyse the correlation and the correlation coefficient (r) illustrate a strong positive linear correlation that is significant, P < 0.05. (C) Histology demonstrated no pathology in adjuvant controls. Representative histology images from C57Bl/6 J Cx 3 cr1 GFP/+ mice immunized with IRBP1-20 illustrate key histopathological features of EAU including vasculitis (D; arrows), granuloma (D; arrowhead), retinal folds (E; arrows), vitritis (E; arrowheads), loss of retinal outer nuclear layer and photoreceptor layer (F; arrow), and cellular infiltrates of the retinal pigment epithelium and choroid (F; arrowheads). IRBP, interphotoreceptor retinoid binding protein.