Figure 5

Microglia/macrophage activation by immunofluorescence staining of Iba-1. A. Morphological changes of microglia/macrophage in ischemic hemispheres over reperfusion courses. Scale bar = 100 μm. The right panels at each time point present a higher magnification of the images shown in squares in the left panels. Scale bar = 50 μm. DAPI was used to show nuclei and vessel at one week. core-i: core infarct area; peri-i: peri-infarct area; V: vessel. B. Quantification of Iba-1 fluorescence (FL) intensity in ischemic hemispheres over reperfusion courses. *P <0.05, ***P <0.001 versus vehicle group, n = 5 in each group. The brain cartoon shows the three images measured by ImageJ that were obtained from the infarct areas, indicated by the squares in red.