LXR activation does not inhibit nuclear translocation of NF-kappaB1 p50. Nuclear extracts from microglial cell line BV2 were isolated following 3 h LPS (10 ng/ml) stimulation. Cells were pretreated with either LXR agonist GW3965 (5 μM) or vehicle (0.05% DMSO). NF-kappaB1 p50 was detected by immunoblotting. Relative density measurements of the p50 bands were normalized to relative beta-actin density. Left figure shows p50 and corresponding beta-actin immunoblots for unstimulated (VC), vehicle pretreated/LPS stimulated (VC/LPS) and GW3965 pretreated/LPS stimulated (GW/LPS) samples from three independent experiments (“Expt”). Right figure shows a summary plot of the normalized relative density measurements of the p50 bands for each condition.