Figure 3

miR-146a targets TRAF6, IRAK1, and IRAK2. CHME3 cells were transfected with 100 pmol miR-146a mimic sequence. Scrambled seed sequence was used as control along with mock treatment as control. The cells were harvested 48 h post transfection. (A) Western blots showing downregulation of TRAF6, IRAK1, and IRAK2 genes upon miR-146a overexpression. (B) Densitometry plot showing downregulation of TRAF6, IRAK1, and IRAK2 genes upon miR-146a overexpression. The overexpressed samples were compared to scramble control for statistical analysis. (C) RT-PCR analysis of miR-146a levels in miR-146a overexpressed cells. The cells were harvested 48 h post transfection. miR-146a levels were determined by RT-PCR by using Taqman probe. RNU24 was used for normalization. All experiments were repeated thrice and are represented as mean ± SE. The fold change is significant where *denotes P < 0.05, **denotes P < 0.005, and ***denotes P < 0.001.