Figure 8

JEV modulates pro-inflammatory cytokine expression and expression of IFIT-1 and IFIT-2. CHME3 cells were infected by JEV, and the effect on expression of pro-inflammatory cytokines and interferon-induced genes (IFIT-1 and IFIT-2) was determined at 12 and 24 h post infection. (A) Graph showing the effect of JEV infection on expression of IL-6 at 12 and 24 h post infection. mRNA levels of IL-6 was determined by RT-PCR. Decrease in IL-6 levels was demonstrated at later time point of 24 h. (B) Graph plot showing increase in TNF-α secretion 12 h post JEV infection by ELISA. The TNF-α level was seen to be decreased after 24 h post infection. (C,D) Graph showing RT-PCR analysis of IFIT-1 (C) and IFIT-2 (D) transcript levels at 12 and 24 h after JEV infection. Increase in IFIT-1 mRNA level was found in early 12 h of infection but later decrease was observed (24 h). All experiments were repeated thrice and are represented as mean ± SE. The fold change is significant where *denotes P < 0.05, **denotes P < 0.005, and ***denotes P < 0.001.