Figure 1

Methamphetamine-mediated up-regulation of σ-1R in primary rat astrocytes. (A) Methamphetamine-mediated induction of σ-1R expression in primary rat astrocytes. Cells were incubated with varying concentrations of methamphetamine (15 μM, 150 μM, and 1.5 mM) for 12 h, followed by collection of protein for assay of σ-1R expression by WB. Representative immunoblots and the densitometric analysis of σ-1R/GAPDH from the three separate experiments are presented. (B) Methamphetamine induced σ-1R expression in a time-dependent manner in primary rat astrocytes. Representative immunoblots and the densitometric analysis of σ-1R/GAPDH from the three separate experiments are presented. (C, D) Effect of methamphetamine on σ-1R RNA in primary rat astrocytes. Total RNA isolated from primary rat astrocytes was subjected to RT-PCR (C) and real-time PCR (D) analyses using primer set specific for σ-1R. All the data are presented as mean ± SD of three individual experiments. *P < 0.05 and **P < 0.01 versus control group.