Figure 2
From: Attenuation of microglial activation in a mouse model of Alzheimer’s disease via NFAT inhibition
FK506 and tat-VIVIT attenuated Aβ-stimulated TNFα secretion by microglia. Microglia were stimulated with 10 μM Aβ1-42 fibrils for 24 h in the absence or presence of 1 μM FK506 or 10 μM tat-VIVIT. (A) Media aliquots were taken and ELISAs were performed to quantitate levels of secreted TNFα. (B) Media was also taken to perform LDH release assays to quantitate cell death. Conditions were performed with eight repeats and averaged (±SD). Graphs are representative of three independent experiments (*p < 0.001 from control).