Figure 4

Effect of knocking-down MEF2D on IL-10 expression. (A) BV2 cells were infected with recombinant lentivirus expressing either control shRNA or shRNA to MEF2D (sh-MEF2D) and then treated with LPS for 24 h. The effectiveness of sh-RNA MEF2D was examined by immunoblotting. (A bottom graph) Relative quantification of MEF2D. Data from three independent experiments were expressed as the mean ± SEM and analyzed by one-way ANOVA (**P < 0.01). (B) The effect of sh-MEF2D on IL-10 mRNA in BV2 cells. BV2 cells infected with sh-MEF2D lentivirus were treated with LPS and analyzed for IL-10 mRNA by qPCR. Data from three independent experiments were expressed as the mean ± SEM and analyzed by two-way ANOVA (**P < 0.01). (C) The effect of sh-MEF2D on the level of secreted IL-10. Culture media from BV2 cells treated as descried under (B) were harvested for ELISA. Data from three independent experiments were expressed as the mean ± SEM and analyzed by two-way ANOVA (**P < 0.01). (D) The effect of sh-MEF2D on TNF-α mRNA in BV2 cells. BV2 cells treated as described under (B) and exposed to LPS for different times were analyzed for TNF-α mRNA by qPCR. Data from three independent experiments were expressed as the mean ± SEM and analyzed by two-way ANOVA (**P < 0.01).