Figure 5

JQ1 suppresses a specific subset of LPS-inducible genes. (A) Heat map representation of the top 50 expression levels of genes that were downregulated (P ≤ 0.01 and fold change ≥1.5) by JQ1 at 2 h (left panel) and (B) 4 h (right panel) after LPS stimulation of two independent BV-2 microglial cultures. Biological replicates (n = 2) for each condition were combined separately, and the heat map were generated with the Multi Experiment Viewer (version 4.8) software. (C and D) UCSC Browser images representing the normalized RNA-Seq read density in JQ1-downregulated inflammatory genes at 2 and 4 h in LPS-stimulated BV-2 microglial cells compared to the control, respectively. LPS, lipopolysaccharide.