Table 1 Sequences for primers
Gene | Gene ID | Primers | Sequences |
|---|---|---|---|
Renin | NM_012642 | Forward | 5′-CAGGAACGATGACCTGTGCAT-3′ |
Reverse | 5′- CAGTGGGTGGTGGGATGTC-3′ | ||
AT1-R | NM_030985 | Forward | 5′-CTCAAGCCTGTCTACGAAAATGAG -3′ |
Reverse | 5′-GTGAATGGTCCTTTGGTCGT -3′ | ||
AT2-R | NM_012494 | Forward | 5′-TGCTGTTGTGTTGGCATTCA-3′ |
Reverse | 5′-ATCCAAGAAGGTCAGAACATGGA-3′ | ||
ACE-1 | NM_012544 | Forward | 5′-TTTGCTACACAAATGGCACTTGT-3′ |
Reverse | 5′-CGGGACGTGGCCATTATATT-3′ | ||
ACE-2 | NM-001012006 | Forward | 5′-TTGAACCAGGATTGGACGAAA-3′ |
Reverse | 5′-GCCCAGAGCCTACGATTGTAGT-3′ | ||
TNF-α | NM_013693 | Forward | 5′-GCATGATCCGCGACGTGGAA-3′ |
Reverse | 5′-AGATCCATGCCGTTGGCCAG-3′ | ||
IL-1β | NM_031512 | Forward | 5′-TGA TGT TCC CAT TAG ACA GC-3′ |
Reverse | 5′-GAG GTG CTG ATG TAC CAG TT-3′ | ||
IL-10 | NM_012854 | Forward | 5′-GTTGCCAAGCCTTGTCAGAAA-3′ |
Reverse | 5′-TTTCTGGGCCATGGTTCTCT-3′ | ||
GAPDH | NM_017008 | Forward | 5′-GCCAAAAGGGTCATCATCTC-3′ |
Reverse | 5′-GGCCATCCACAGTCTTCT-3′ |