Effects of miR-15b and miR-16 on Akt phosphorylation and cleaved caspase 3 in hyperglycemia. REC were cultured in normal glucose (NG, 5 mM) or high glucose (HG, 25 mM). (A) Western blot results for phosphorylated Akt to total Akt on retinal endothelial cells (REC). Overexpression of microRNA (miR)-15b and miR-16 increased the levels of Akt phosphorylation, which was reduced in the control HG condition. A representative blot is shown. (B) Enzyme-linked immunosorbent assay (ELISA) results for cleaved caspase 3. miR-16 and miR-15b/16 significantly reduced the level of cleaved caspase 3 in hyperglycemia. #
P < 0.05 versus NG, *P < 0.05 versus HG, N = 4; data are mean ± S.E.M.