Figure 2
From: Endogenous antibodies contribute to macrophage-mediated demyelination in a mouse model for CMT1B
Lack of evidence for complement deposition in peripheral nerve fibers of P0het mice. (A) Immunocytochemical staining against C3 on single teased-fiber preparations. Complement deposition is detectable in teased fibers from crushed wildtype sciatic nerves (3 days post crush) but not in nonlesioned sciatic nerves from 6-month-old P0wt and P0het mice. Corresponding phase contrast (PC) micrographs are also shown. (B) Immunohistochemical staining against C3 (green) and α-Bungarotoxin (αBTX, red) on diaphragm cross section. Complement deposition is detectable at presynaptic terminals. Blue profiles in merged micrographs are DAPI-labeled nuclei. Scale bars, 5 μm.