Figure 4

Cat H induced microglial activation and promoted release of proinflammatory cytokines in vitro. The level of nitrite in the media of BV2 cells following various concentrations of Cat H treatments for 24 h was shown in (A). Release of cytokines in the media of BV2 cells treated with various concentrations of Cat H was measured by ELISA and shown in (B). The effect of Cat H antibody on release of cytokines in BV2 cells was shown in (C) (Cat H concentration is 2 ng/μL). Data were expressed as mean + SEM from three independent experiments. ^a,b P < 0.01 vs control; ^c P < 0.05 vs a in A; ^a,c P < 0.05 vs control; ^b,d P < 0.05 vs a, c in B. **: P < 0.01 in C.