The time course of i.t. Ad-PTEN effects on nociceptive behaviors in CCI rats. Ad-PTEN (i.t) prevented CCI-induced development of pain behaviors with the ipsilateral hindpaw in neuropathic rats up to 14 days post injury, including thermal hyperalgesia (A), mechanical allodynia (B), cold allodynia (C), and weight-bearing deficits (D). Each point represents the mean ± SEM with six rats per group. *P < 0.05 compared with CCI plus i.t. vehicle group. Ad-GFP, adenovirus-mediated green fluorescent protein; Ad-PTEN, adenovirus-mediated phosphatase and tensin homolog deleted from chromosome 10; CCI, chronic constriction injury; i.t., intrathecal.