Transplantation of MSCs increased the levels of zonula occludens-1 (ZO-1) and claudin-5. Immunofluorescence analysis of ZO-1 and western blotting analysis of ZO-1 and claudin-5. Immunofluorescence analysis of ZO-1 (A) showed that treatment of MSCs increased the levels of tight junction protein compared with the PBS-treated group 72 h after ICH. Western blotting analysis of ZO-1 (B, C) and claudin-5 (B, D) showed similar results in that transplantation of MSCs upregulated the levels of tight junctions compared with the PBS-treated group 24 and 72 h after ICH. n = 6 in each time point per group. Data are presented as the mean ± SD. Bar = 50 μm. *P < 0.05; **P < 0.01. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MSCs, mesenchymal stem cells; PBS, phosphate-buffered saline.