Transplantation of MSC decreased the levels of inducible nitric oxide synthase (iNOS). Immunofluorescence and western blotting analysis of iNOS. Immunofluorescence analysis of iNOS (A) showed that treatment with MSCs decreased the levels of iNOS compared with the PBS-treated group 72 h after ICH. Western blotting (B, C) analysis of iNOS showed similar results in that transplantation of MSCs downregulated the levels of iNOS compared with the PBS-treated group 24 and 72 h after ICH. n = 6 in each time point per group. Data are presented as the mean ± SD. Bar = 50 μm. *P < 0.05; **P < 0.01. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; iNOS, inducible nitric oxide synthase; MSCs, mesenchymal stem cells; PBS, phosphate-buffered saline.