Transplantation of MSCs decreased the levels of 3-nitrotyrosine (3-NT). Immunofluorescence and western blotting analysis of 3-NT. Immunofluorescence analysis of 3-NT (A) showed that the treatment with MSCs decreased the levels of 3-NT compared with the PBS-treated group 72 h after ICH. Western blotting (C, D) analysis of 3-NT showed the similar results in that transplantation of MSCs downregulated the levels of 3-NT compared with the PBS-treated group 24 and 72 h after ICH. The double labeling of 3-NT and ZO-1 indicated that 3-NT and vascular damage are closely related (B).
n = 6 in each time point per group. Data are presented as the mean ± SD. Bar = 50 μm. *P < 0.05; **P < 0.01. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; MSCs, mesenchymal stem cells; PBS, phosphate-buffered saline.