Figure 3

Inhibition of disease progression in GFAPγR1Δ mice correlates with decreased demyelination and axonal damage. Spinal cord sections of GFAPγR1Δ mice treated with α-β-gal and α-IL-6 mAbs at day 32 post immunization stained with LFB (A) or anti-APP (C) to assess demyelination and axonal damage, respectively. Representative sections from three experiments with three individual mice per group per experiment. In (A), areas of demyelination are shown with arrows. Bar = 200 μm. (B) Percentage area of demyelination in spinal cord white matter calculated by analysis of transverse sections at six separate levels per mouse. Data represent the mean ± SEM of seven to nine individual mice per group from two separate experiments. APP⁺-damaged axons (C) shown with arrows. Bar = 100 μm. (D) Number of APP⁺ axons per 100-μm² area of demyelination analyzed in GFAPγR1Δ mice treated with α-β-gal and α-IL-6 mAb at day 32 post immunization. Data represent the mean ± SEM of four to five individual mice per group from two separate experiments. Statistical differences determined by a two-tailed unpaired t test with *P < 0.05.