Figure 5

Inflammatory leukocytes during progressive EAE are reduced by IL-6 blockade. (A) Total number of bone marrow-derived inflammatory cells (CD45^hi). Data represent the mean ± SEM of two separate experiments with n = 4 per group per experiment. Statistics were calculated with one-way ANOVA with Bonferroni post-test with **P < 0.01; ***P < 0.001. (B) Inflammatory cells within the spinal cord sections of GFAPγR1Δ mice treated with α-β-gal or α-IL-6 mAb at day 32 post immunization identified with H&E. Inflammatory cells were limited to lesions and were both perivascular (arrows) and within the parenchyma. Bar = 50 μm. Representative sections from two experiments with four individual mice per group per experiment. (C) CD4⁺ T cells (CD45^hiCD4⁺) within the CNS of WT, GFAPγR1Δ + α-β-gal, and GFAPγR1Δ + α-IL-6 mice analyzed by flow cytometry at day 32 post immunization. Data represent the mean ± SEM of two separate experiments with n = 4 per group per experiment.