Figure 7

Proliferation of inflammatory cells during progressive EAE is reduced by anti-IL-6 treatment. (A) Proliferation of CD45^hi cells within the CNS of WT and GFAPγR1Δ mice during acute EAE (day 20 post immunization) or WT, GFAPγR1Δ + α-β-gal, and GFAPγR1Δ + α-IL-6 mice during chronic EAE (day 32 post immunization) measured by BrdU staining and flow cytometry. Four individual mice were analyzed per group per time point. (B) Percentage of BrdU-positive cells within CD4⁺ cells analyzed at day 32 post immunization in WT and GFAPγR1Δ mice treated with α-β-gal or α-IL-6 mAb. Data represent the mean ± SEM of two separate experiments with n = 4 per group per experiment. Statistics were calculated with one-way ANOVA with Bonferroni post-test with *P < 0.05.