Expression of inflammatory genes in mouse cortical slices incubated on various times. IL-1β, TNF-α, CCL2, CCL3, CCL4, and PTGS2 mRNAs were measured at 0, 0.5, 4.5, 6, and 24 h in culture. Target mRNAs were normalized to β-actin mRNA as an endogenous control reference, and differences among the groups were tested with Dunnett’s multiple comparison test. n = 6 per time point. *Means ± SEM differ P < 0.05 relative to time zero; **means ± SEM differ P < 0.01 relative to time zero. CCL2, chemokine ligand 2; CCL3, chemokine ligand 3; CCL4, chemokine ligand 4; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL-1β, interleukin-1β; PTGS2, prostaglandin-endoperoxide synthase 2; TNF-α, tumor necrosis factor-α.