Early inflammatory mediator gene expression in two models of traumatic brain injury: ex vivo cortical slice in mice and in vivo cortical impact in piglets

Table 1 Primer sequences for real-time qPCR analysis of piglet gene expression

mRNA	Swine primer sequence (5′ - 3′)	Amplicon ^a (bp)	Accession # ^b
IL-1β F^c	AGGCACAAAGGCCATTCAGT	102	NM_001005149.1
IL-1β R^c	ACTTCCTTGGCAGGTTCAGG
TNF-α F	CATCTACCTGGGAGGGGTCT	109	NM_214022.1
TNF-α R	ACCTGCCCAGATTCAGCAAA
CCL2 F	CACCAGCAGCAAGTGTCCTA	86	NM_214214.1
CCL2 R	CCCACTTCTGCTTGGGTTCT
CCL3 F	TATTTTGAGACCAGCAGCCAGT	127	NM_001009579.1
CCL3 R	CATTCAGCTCCAGGTCAGAGAT
CCL4 F	TTCACATACACCGTGCGGAA	148	NM_213779.1
CCL4 R	ACTCCTGGACCCAGTCATCA
PTGS2 F	CCAGCACTTCACCCATCAGT	132	NM_214321.1
PTGS2 R	AGGCGCAGTTTATGCTGTCT
β-actin F	CAAGCAGGAGTACGACGAGT	145	XM_003357928.2
β-actin R	GGCTGGCATGAGGTGTGTA
GAPDH F	AAGGTCGGAGTGAACGGATT	149	NM_001206359.1
GAPDH R	CCGTGGGTGGAATCATACTGG

^aPredicted number of base pairs (bp) in PCR product; ^bNCBI reference sequence; ^cF, forward primer; R, reverse primer. CCL2, chemokine ligand 2; CCL3, chemokine ligand 3; CCL4, chemokine ligand 4; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; IL-1β, interleukin-1β; PTGS2, prostaglandin-endoperoxide synthase 2; TNF-α, tumor necrosis factor-α.

ISSN: 1742-2094