Fig. 6

EA inhibited microglia activation and inflammatory responses in A53T mice. a Immunofluorescent images illustrating effects of EA on Iba1 immunostaining in the SN of A53T and nTg control mice. Iba1-containing microglia was visualized by Iba-1 immunostaining. b and c Effects of EA on TNF-α (b) and IL-1β (c) expression in the midbrain (upper panels) and striatum (lower panels) of A53T mice and nTg control mice. TNF-α and IL-1β protein levels were assessed by ELISA. Note that EA significantly reduced TNF-α expression in the midbrain and striatum and IL-1β expression in the striatum of A53T mice. Data are expressed as means ± SEM. *P < 0.05, **P < 0.01 vs. nTg group. #P < 0.05, ###P < 0.001 vs. A53T group