Fig. 7

EA protected DA neurons in the SNpc and motor neurons in the spinal cord in A53T mice. a Representative immunohistochemical photomicrographs showing TH immunostainings in the SNpc of A53T and nTg control mice following EA treatment. Scale bar, 200 μm. b Quantification of TH-positive dopaminergic neurons in the SNpc of A53T and nTg control mice following EA treatment. The TH neurons were counted by stereological analysis. c Effects of EA on the striatal DA content. Striatal DA levels were assessed by HPLC. d Effects of EA on TH protein levels in the midbrain. TH proteins were detected by immunoblot analysis. Representative immunoblots are shown above the quantified data. e Representative images show NeuN staining in the ventral horn of lumbar spinal cord of control nTg mice, EA+ nTg mice, A53T mice, and EA+ A53T mice. Scale bar, 200 μm. f These images are the enlarged views from areas indicated in the (e). Scale bar, 200 μm. Data are expressed by means ± SEM (n = 5 per group). *P < 0.05, **P < 0.01, ***P < 0.001 vs. nTg group. #P < 0.05, ##P < 0.01 vs. A53T group