Figure 3

Dose-dependent COX-2 inhibition induced by MH in different biological matrices. (A) MH-mediated inhibition of hCOX-2 using 10 μM of AA (black), AEA (blue), and 2-AG (red) as substrates. MH-mediated inhibition of PGE₂ and PGE₂-GE formation in (B) RAW264.7 cell homogenate and (C) intact cells after 12 h of incubation with LPS (1 mg mL⁻¹). Homogenates and intact cells were incubated for 30 min with 10 μM of 2-AG after the pre-treatment (30 min) with different concentrations of MH or vehicle. ^* P < 0.05, ^** P < 0.01 PGE₂ vs. PGE₂-GE.