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Table 2 Summary of antibodies used for immunohistochemistry

From: Absence of toll-like receptor 4 (TLR4) extends survival in the hSOD1G93A mouse model of amyotrophic lateral sclerosis

Antibody Manufacturer Dilution In combination with
Rat anti-mouse TLR4 R & D Systems 1:500 GFAP, Iba-1 and ChAT
Rabbit anti-mouse HMGB1 Abcam 1:1,000 GFAP, CD11b and ChAT
Mouse anti-mouse GFAP BD Biosciences 1:1,000 TLR4
Rabbit anti-mouse Iba-1 Wako 1:400 TLR4
Goat anti-mouse ChAT Chemicon 1:100 TLR4
  1. TLR4, toll-like receptor 4; HMGB1, high-mobility group box 1; GFAP, glial fibrillary acidic protein; ChAT, choline acetyltransferase; Iba-1, ionised calcium-binding adapter molecule-1.