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Table 2 Summary of antibodies used for immunohistochemistry

From: Absence of toll-like receptor 4 (TLR4) extends survival in the hSOD1G93A mouse model of amyotrophic lateral sclerosis

Antibody

Manufacturer

Dilution

In combination with

Rat anti-mouse TLR4

R & D Systems

1:500

GFAP, Iba-1 and ChAT

Rabbit anti-mouse HMGB1

Abcam

1:1,000

GFAP, CD11b and ChAT

Mouse anti-mouse GFAP

BD Biosciences

1:1,000

TLR4

Rabbit anti-mouse Iba-1

Wako

1:400

TLR4

Goat anti-mouse ChAT

Chemicon

1:100

TLR4

  1. TLR4, toll-like receptor 4; HMGB1, high-mobility group box 1; GFAP, glial fibrillary acidic protein; ChAT, choline acetyltransferase; Iba-1, ionised calcium-binding adapter molecule-1.
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Journal of Neuroinflammation

ISSN: 1742-2094