Figure 3

Effect of ATK on CEC-induced increased activity/expression of cPLA2, 5-LOX, and COX-2. Expression of cPLA2, 5-LOX, and COX-2 and activation of cPLA2 and 5-LOX (in terms of increased levels of their phosphorylation) were measured in the spinal cord tissue from epicenter (E) and rostral (R) regions at 24 h after CEC using western analysis (A) and its densitometry (B). CEC had no effect on the expression of cPLA2, whereas it increased the expression of p cPLA2, p-5-LOX, 5-LOX, and COX-2 in the epicenter region. While ATK treatment reduced the expression of pcPLA2, the treatment had no significant effects on the expression of p-5-LOX, 5-LOX, and COX-2. Data are presented as mean ± SD (n = 5). Statistical significance was evaluated between epicenter (E) and rostral (R) regions in the same group, and E was compared between CEC + VEH and CEC + ATK groups. ***p < 0.001 vs. R of the spinal cord tissue from the same group, +++p < 0.001 vs. E of the spinal cord from CEC + ATK.