Fig. 5

Effects of CXCR2 or CXCL1 deficiency on the expression of P-selectin, E-selectin, and adhesion molecules in vivo and on BBB permeability. a The protein expression of P-selectin, E-selectin, VCAM-1, and ICAM-1 (4 h after i.c.v. saline injection) in the saline-treated control group of WT mice and LPS-treated (4 h after i.c.v. LPS injection) WT, CXCR2^−/−, and CXCL1^−/− mice was determined via Western blot analysis. Effects of CXCR2 or CXCL1 deficiency on P-selectin (b), VCAM-1 (c), and ICAM-1 (d) expression in the brain. e Western blotting analysis of the albumin levels in the brains of WT and CXCR2^−/− mice 4, 12, and 24 h after the intraventricular injection of LPS was performed. Optical densities were determined using a computer imaging analysis system. n = 5 mice per group. **P < 0.01; ***P < 0.001