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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: TANK-binding kinase 1 (TBK1) modulates inflammatory hyperalgesia by regulating MAP kinases and NF-κB dependent genes

Fig. 3

Inflammatory hyperalgesia in TNFR−/− and TBK1−/−/TNFR−/− mice. a Left panel: time course of mechanical hyperalgesia in wild type (black triangle), TNFR−/− (dark grey square), and TBK1−/−/TNFR−/− (light grey circle) mice after injection of 10 mg/ml (20 μl) zymosan A into a hind paw. The diagram shows the delta paw withdrawal latencies (ΔPWL) in response to mechanical stimulation as assessed with a Dynamic Plantar Aesthesiometer (n = 6 mice/group), repeated measure ANOVA with Bonferroni post-hoc analysis, *P < 0.05 and **P < 0.01. Upper right panel: comparison of the area under the paw withdrawal latency versus time curve between wild type (black column), TNFR−/− (dark grey column), and TBK1−/−/TNFR−/− (light grey column) mice 3 to 48 h after zymosan A injection. Univariate ANOVA with Bonferroni post-hoc analysis # P < 0.05 significant mean difference between the respective genotypes. Lower right panel: determination of the paw weight of wild type (black column), TNFR−/− (dark grey column), and TBK1−/−/TNFR−/− (light grey column) mice 48 h after zymosan A injection. b Left panel: time course of the licking behavior in wild type (black triangle), TNFR−/− (dark grey square), and TBK1−/−/TNFR−/− (light grey circle) mice (n = 8 mice/group) after injection of formalin (5 %, 20 μl) into the hind paw. Formalin was injected at time ‘0’, and the time spent licking the injected paw was measured at 5 min intervals for 45 min. Right panel: statistical analysis of phase I (0–5 min) and phase II (15–45 min) between wild type (black column), TNFR−/− (dark grey column), and TBK1−/−/TNFR−/− (light grey column). Univariate ANOVA with Bonferroni post-hoc analysis ## P < 0.01 significant mean difference between the respective genotypes

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