Fig. 3

Inflammatory hyperalgesia in TNFR^−/− and TBK1^−/−/TNFR^−/− mice. a Left panel: time course of mechanical hyperalgesia in wild type (black triangle), TNFR^−/− (dark grey square), and TBK1^−/−/TNFR^−/− (light grey circle) mice after injection of 10 mg/ml (20 μl) zymosan A into a hind paw. The diagram shows the delta paw withdrawal latencies (ΔPWL) in response to mechanical stimulation as assessed with a Dynamic Plantar Aesthesiometer (n = 6 mice/group), repeated measure ANOVA with Bonferroni post-hoc analysis, *P < 0.05 and **P < 0.01. Upper right panel: comparison of the area under the paw withdrawal latency versus time curve between wild type (black column), TNFR^−/− (dark grey column), and TBK1^−/−/TNFR^−/− (light grey column) mice 3 to 48 h after zymosan A injection. Univariate ANOVA with Bonferroni post-hoc analysis ^# P < 0.05 significant mean difference between the respective genotypes. Lower right panel: determination of the paw weight of wild type (black column), TNFR^−/− (dark grey column), and TBK1^−/−/TNFR^−/− (light grey column) mice 48 h after zymosan A injection. b Left panel: time course of the licking behavior in wild type (black triangle), TNFR^−/− (dark grey square), and TBK1^−/−/TNFR^−/− (light grey circle) mice (n = 8 mice/group) after injection of formalin (5 %, 20 μl) into the hind paw. Formalin was injected at time ‘0’, and the time spent licking the injected paw was measured at 5 min intervals for 45 min. Right panel: statistical analysis of phase I (0–5 min) and phase II (15–45 min) between wild type (black column), TNFR^−/− (dark grey column), and TBK1^−/−/TNFR^−/− (light grey column). Univariate ANOVA with Bonferroni post-hoc analysis ^## P < 0.01 significant mean difference between the respective genotypes