Fig. 2
From: Inhibition of NADPH oxidase activation reduces EAE-induced white matter damage in mice
MOG-induced ROS production and p47phox expression are reduced by apocynin. ROS production in the spinal cord was detected by dHEt fluorescence staining 21 days after the first immunization. Mice were sacrificed 3 h after dHEt injection. a EAE mice (EAE + vehicle, n = 3) showed substantially increased Et fluorescence intensity in the white matter of the spinal cord. Apocynin (EAE + apocynin, n = 3) treatment showed substantial reduction of this Et fluorescence intensity in EAE mice. b The square area in the low magnification of panel a was enlarged four hundred times and illustrated in the right-hand panel. Scale bar represents 50 μm. c The graph represents Et fluorescence intensity as mean gray value (Image J). Data are mean ± sem (n = 3). *p < 0.05 compared with the apocynin-treated group. d To test whether apocynin treatment influences NADPH oxidase activation in EAE mice, NADPH oxidase subunit (p47phox) and neurons (MAP2) were immunohistochemically stained. Sham-operated spinal cord section showed only weak p47phox expression. A prominent increase in p47phox expression was seen throughout the spinal cord 21 days after MOG injections (EAE + vehicle, n = 3). However, the expression of p47phox was reduced by apocynin treatment (EAE + apocynin, n = 3) in EAE mice. e The square area in the low magnification of d panel was enlarged four hundred times and illustrated in the right-hand panel. Scale bar represents 50 μm. f The graph represents p47phox fluorescence intensity as mean gray value (Image J). Data are mean ± sem (n = 3). *p < 0.05 compared with apocynin-treated group