Fig. 2From: Crosstalk between macrophages and astrocytes affects proliferation, reactive phenotype and inflammatory response, suggesting a role during reactive gliosis following spinal cord injuryEffects of macrophage-derived soluble factors on spinal cord organotypic cultures. a Whole-slice MTT shows increased cellular respiration following incubation with M1-conditioned, but not M2-conditioned medium. b Cord cultures incubated for 72 h with conditioned medium of M1, but not M2, macrophages show increased numbers of dividing cells, as shown by EdU nucleotide incorporation. c The proportion of EdU incorporating cells that also express GFAP is not changed in experimental conditions. d–o Immunohistochemistry on cryosectioned slice cultures shows a significant fraction of dividing cells are GFAP+ astrocytesBack to article page