Fig. 1
IVIG bind specifically to the Schwann cell surface. a–g Living Schwann cells were decorated for 24 h with either dialysed control buffer (ctrl) (a–c), 20 mg/ml dialysed IVIG (d–f), or dialysed F(ab′)2 fragments, corresponding to 5 mg/ml IVIG (g). After fixation, the cells were stained against human F(ab′)2 (red) and human Fc parts (green), and nuclei were labeled with DAPI (blue). IVIG and F(ab′)2 fragments binding could be detected by means of co-immunostaining (a, d, g) or single staining against human F(ab′)2 (b, e) or human Fc fragments (c, f). Application of dialysed control buffer (a–c) resulted in no staining. Scale bar, 50 μm. n = 8 for a–f, n = 4 for g