Fig. 6

Inhibitory effect of BV on LPS-induced NF-κB DNA-binding activity and on NF-κB-related protein in mice brain. Activation of NF-κB was investigated using EMSA as described in the Methods section. Nuclear extracts were subjected to DNA-binding reaction with ³²P end-labeled oligonucleotide specific to NF-κB. Specific DNA binding of the NF-κB complex is indicated by an arrow (a). The expression of IκB, p-IκB, p50, and p65 were detected by western blotting using specific antibodies in the mice brain. Each blot is representative of three experiments (b). Structural interaction between melittin and p50 of NF-κB subunit. Melittin structure is c. Pull-down assay identifies an interaction between the melittin and p50. Melittin was conjugated with melittin-activated Sepharose 6B (d). The docking model of melittin with p50 is as described in the Materials and Methods section (e)