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Fig. 1 | Journal of Neuroinflammation

Fig. 1

From: Pro-inflammatory TNFα and IL-1β differentially regulate the inflammatory phenotype of brain microvascular endothelial cells

Fig. 1

Analysis of pro-inflammatory IL-1β and TNFα activation of brain endothelial hCMVECs on cellular adhesion measured using xCELLigence Biosensor technology. a The net endothelial adhesion (cell index) was measured using xCELLigence across a 210-h period. Endothelial cells were treated with IL-1β (5 ng/mL; green curve) and TNFα (5 ng/mL; blue curve) 24 h after seeding. Cytokine addition is indicated by the blue arrow, whereas the red arrows highlight the time points when conditioned media was collected (from parallel plates). The control (vehicle cells) is indicated by the red curve. b The curves represent the mean of four individual well ± SD of a representative experiment

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