Transcript analysis of laser capture microdissected white matter astrocytes and higher phenol sulfotransferase 1A1 expression during autoimmune neuroinflammation

Table 3 Summary of TLDA analysis

	Mix tissue	LCM-astrocytes
	Ct value	dCt Con	dCt EAE	Fold increase (2^−ddCt)
Gene symbol		Detected in all EAE samples at poor to good levels
Arg1	25.1	14.3 ± 1.0	13.2 ± 1.5	2.2	NS	¥
Ccl2	23.3	12.0 ± 0.5	3.5 ± 0.6	349	*	¥
Ccl3	23.9	11.1 ± 2.7	11.8 ± 4.4	0.6	NS
Ccl5	18.5	12.0 ± 2.5	4.4 ± 1.1	199	*	¥
Ccl7	23.9	12.5 ± 2.1	4.6 ± 0.4	231	*	¥
Cxcl9	21.3	13.0 ± 1.7	4.9 ± 0.9	270	*	¥
Cxcl10	21.7	13.8 ± 1.1	6.1 ± 0.6	214	*	¥
Cxcl16	21.4	9.8 ± 1.9	5.0 ± 0.5	28	*
Dbi	12.8	0.7 ± 0.2	−0.2 ± 0.2	1.8	*
Gfap	24.7	9.7 ± 1.2	11.9 ± 3.0	0.2	NS
Lcn2	13.7	10.5 ± 2.9	2.5 ± 0.4	253	*
Mbp	22.1	7.9 ± 1.7	7.4 ± 1.5	1.4	NS
Serping1	16.5	11.0 ± 2.5	5.4 ± 0.3	48	*
Spp1	23.2	13.1 ± 1.1	7.3 ± 1.0	54	*
Sult1a1	18.1	10.5 ± 0.5	7.5 ± 0.7	7.7	*
Tnf	33.3	10.2 ± 0.8	13.9 ± 1.5	0.1	NS
Tspo	17.6	10.7 ± 2.8	5.4 ± 1.1	31	*
		Detected at poor levels in some EAE samples
Il1b	26.2	Detected only in one EAE sample
Il15	23.4	Detected only in two EAE samples
Il15ra	22.9	Detected only in two EAE samples
Tnfrsf9	28.8	Detected only in one EAE sample
Tnfsf9	29.3	Detected only in one EAE sample
		Detected at very poor levels in all samples
Cxcl12	24.6	13.9 ± 0.8	14.6 ± 1.7
Tgfb1	33.6	13.9 ± 1.1	18.2 ± 0.5
Ugt8a	30.1	12.7 ± 1.7	17.7 ± 0.4
		Undetected in all samples
Sult1c2	25.0		Undetected
Sult1d1	22.8		Undetected
Sult1e1	25.1		Undetected
Sult2a1	18.3		Undetected
Sult2b1	23.2		Undetected
Sult3a1	19.7		Undetected
Ugt1a1	24.2		Undetected
Ugt2a3	21.6		Undetected
Ugt2b5	20.2		Undetected
Ugt2b34	23.3		Undetected
Ugt3a1	23.3		Undetected

Ct value for mixed tissue which contained liver, spleen, and brain cDNA indicated that the probes were effective in detecting the transcript. dCt values for control and EAE correspond to the Ct value of the target gene minus the Ct geometric mean of the two housekeeping genes. For LCM-astrocyte samples, dCt values are expressed as mean ± sem. Transcripts showing significant EAE-induced increased expression are in italics
NS p > 0.05, ¥ undetected in two of the three control samples (Ct value fixed to 38)
*p < 0.05

ISSN: 1742-2094