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Table 3 Summary of TLDA analysis

From: Transcript analysis of laser capture microdissected white matter astrocytes and higher phenol sulfotransferase 1A1 expression during autoimmune neuroinflammation

  Mix tissue LCM-astrocytes     
  Ct value dCt Con dCt EAE Fold increase (2−ddCt)   
Gene symbol   Detected in all EAE samples at poor to good levels
Arg1 25.1 14.3 ± 1.0 13.2 ± 1.5 2.2 NS ¥
Ccl2 23.3 12.0 ± 0.5 3.5 ± 0.6 349 * ¥
Ccl3 23.9 11.1 ± 2.7 11.8 ± 4.4 0.6 NS  
Ccl5 18.5 12.0 ± 2.5 4.4 ± 1.1 199 * ¥
Ccl7 23.9 12.5 ± 2.1 4.6 ± 0.4 231 * ¥
Cxcl9 21.3 13.0 ± 1.7 4.9 ± 0.9 270 * ¥
Cxcl10 21.7 13.8 ± 1.1 6.1 ± 0.6 214 * ¥
Cxcl16 21.4 9.8 ± 1.9 5.0 ± 0.5 28 *  
Dbi 12.8 0.7 ± 0.2 −0.2 ± 0.2 1.8 *  
Gfap 24.7 9.7 ± 1.2 11.9 ± 3.0 0.2 NS  
Lcn2 13.7 10.5 ± 2.9 2.5 ± 0.4 253 *  
Mbp 22.1 7.9 ± 1.7 7.4 ± 1.5 1.4 NS  
Serping1 16.5 11.0 ± 2.5 5.4 ± 0.3 48 *  
Spp1 23.2 13.1 ± 1.1 7.3 ± 1.0 54 *  
Sult1a1 18.1 10.5 ± 0.5 7.5 ± 0.7 7.7 *  
Tnf 33.3 10.2 ± 0.8 13.9 ± 1.5 0.1 NS  
Tspo 17.6 10.7 ± 2.8 5.4 ± 1.1 31 *  
   Detected at poor levels in some EAE samples   
Il1b 26.2 Detected only in one EAE sample   
Il15 23.4 Detected only in two EAE samples   
Il15ra 22.9 Detected only in two EAE samples   
Tnfrsf9 28.8 Detected only in one EAE sample   
Tnfsf9 29.3 Detected only in one EAE sample   
   Detected at very poor levels in all samples  
Cxcl12 24.6 13.9 ± 0.8 14.6 ± 1.7    
Tgfb1 33.6 13.9 ± 1.1 18.2 ± 0.5    
Ugt8a 30.1 12.7 ± 1.7 17.7 ± 0.4    
   Undetected in all samples    
Sult1c2 25.0   Undetected    
Sult1d1 22.8   Undetected    
Sult1e1 25.1   Undetected    
Sult2a1 18.3   Undetected    
Sult2b1 23.2   Undetected    
Sult3a1 19.7   Undetected    
Ugt1a1 24.2   Undetected    
Ugt2a3 21.6   Undetected    
Ugt2b5 20.2   Undetected    
Ugt2b34 23.3   Undetected    
Ugt3a1 23.3   Undetected    
  1. Ct value for mixed tissue which contained liver, spleen, and brain cDNA indicated that the probes were effective in detecting the transcript. dCt values for control and EAE correspond to the Ct value of the target gene minus the Ct geometric mean of the two housekeeping genes. For LCM-astrocyte samples, dCt values are expressed as mean ± sem. Transcripts showing significant EAE-induced increased expression are in italics
  2. NS p > 0.05, ¥ undetected in two of the three control samples (Ct value fixed to 38)
  3. *p < 0.05