Fig. 2

β-LAP suppressed the LPS-induced expression and enzymatic activity of MMP-3, MMP-8, and MMP-9, whereas it enhanced TIMP-2 expression. BV2 cells (a) or primary microglia (b) were pretreated with β-LAP (0.5, 1, and 2 μM, for 1 h), followed by LPS (100 or 10 ng/ml), and total RNA was isolated at 6 h after LPS treatments. The mRNA expressions of MMPs and TIMP-2 were analyzed by RT-PCR. Representative gels are shown in the left panel, and quantification data are shown in the right panel (n = 3). c Western blot analysis was performed using conditioned medium (CM) or cell lysates of BV2 cells pretreated with β-LAP (0.5, 1, and 2 μM, for 1 h), followed by LPS (100 ng/ml) for 16 h. Levels of MMP-3, MMP-8, and MMP-9, and TIMP-2 protein expression were normalized using β-actin and were expressed as relative fold changes in comparison with control samples. d The enzymatic activities of MMPs in the CM were detected using MMP activity assay kits. BV2 cells were pretreated with β-LAP (0.5, 1, and 2 μM, for 1 h), followed by LPS (100 ng/ml, for 24 h), and the CM was collected to measure MMP activity. MMP activity units were expressed as a change in fluorescence intensity. Values are expressed as the means ± SEM for three independent experiments. *P < 0.05, significantly different from the LPS-treated group