Fig. 6

Stroke-induced neutrophil migration into the liver is impaired in mice fed a high-fat diet. Male C57BL/6 mice (8-week-old) were maintained on either a control or high-fat diet for 6 months when 30-min MCAo was performed. Representative photomicrographs illustrating immunohistochemical detection of neutrophils in the liver of naïve control (a) and high-fat fed (b) mice, and after MCAo in control (c) and high-fat-fed (d) mice. Scale bars, 100 μm. e The average number of neutrophils/area was assessed after 24-h reperfusion. For this group of animals, the data for volume of ischaemic damage at 24 h is illustrated in Fig. 1e. Data are shown as mean ± standard error of the mean (SEM). n = 5–6/group. *P < 0.05, ***P < 0.001. Two-way ANOVA with Bonferroni’s